Constraint-Based Modeling of Yeast Mitochondria Reveals the Dynamics of Protein Import and Iron-Sulfur Cluster Biogenesis
نویسندگان
چکیده
Highlights • Reconstruction of mitochondrial protein import and cofactor metabolism in the model eukaryote Saccharomyces cerevisiae Quantification proton motive force shows significant energy cost metabolite transport Protein activity depends on growth rate respiratory iron-sulfur cluster requirements show dependence
منابع مشابه
The Rieske Iron-Sulfur Protein: Import and Assembly into the Cytochrome bc1 Complex of Yeast Mitochondria
The Rieske iron-sulfur protein, one of the catalytic subunits of the cytochrome bc(1) complex, is involved in electron transfer at the level of the inner membrane of yeast mitochondria. The Rieske iron-sulfur protein is encoded by nuclear DNA and, after being synthesized in the cytosol, is imported into mitochondria with the help of a cleavable N-terminal presequence. The imported protein, besi...
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Iron-sulfur (Fe-S) clusters are essential for numerous biological processes, including mitochondrial respiratory chain activity and various other enzymatic and regulatory functions. Human Fe-S cluster assembly proteins are frequently encoded by single genes, and inherited defects in some of these genes cause disease. Recently, the spectrum of diseases attributable to abnormal Fe-S cluster bioge...
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Monothiol glutaredoxins play a crucial role in iron-sulfur (Fe/S) protein biogenesis. Essentially all of them can coordinate a [2Fe-2S] cluster and have been proposed to mediate the transfer of [2Fe-2S] clusters from scaffold proteins to target apo proteins, possibly by acting as cluster transfer proteins. The molecular basis of [2Fe-2S] cluster transfer from monothiol glutaredoxins to target p...
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ژورنال
عنوان ژورنال: Social Science Research Network
سال: 2021
ISSN: ['1556-5068']
DOI: https://doi.org/10.2139/ssrn.3813277